ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2017, Vol. 48 ›› Issue (3): 577-584.doi: 10.11843/j.issn.0366-6964.2017.03.023

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The Expression of Octamer-binding Transcription Factor 4 Gene (Oct 4) in Yak (Bos grunneins) Oocytes and Preimplantation Embryos Produced by in vitro Fertilization

ZHAO Tian，LI Gu-yue，PAN Yang-yang，LI Yue-qin，CHEN Ping，WEN Ze-xing，CUI Yan， YU Si-jiu*   

  1. (Technology and Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep & Goat, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070，China)
  • Received:2016-08-22 Online:2017-03-23 Published:2017-03-23

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Abstract:

The aim of this study was to explore the effect of octamer-binding transcription factor 4(Oct 4) during yak oocytes maturation and embryos development, which was achieved by examined the expression of transcription factors Oct 4 at different stages. In this study, yak cumulus-oocyte complexes (COCs) were collected and matured in vitro, the embryos were produced by the method of in vitro fertilization (IVF) and the development competence of oocytes and embryos at different stages were evaluated. The expression and distribution of Oct 4 in the level of mRNA and protein in COCs and embryos at different stages were detected by quantitative real-time PCR (qRT-PCR) and indirect immunofluorescence. The results showed as follows: 1) The rates of embryos developed to stages of 2-4-cells, 5-8-cells, 9-16-cells and blastocyst were (64.66±1.76)%, (43.33±1.76)%, (24.66±2.40)%, (9.33±0.67)%, respectively. 2) The expression of Oct 4 gene could be detected at all stages during yak oocytes maturation and embryos development, which was highest in 9-16-cells embryos, followed by blastocyst, 2-4-cells embryos and 5-8-cells embryos, while it were lowest in immature COCs and mature COCs.3) The result of indirect immunofluorescence method showed that the protein of Oct 4 was mainly distributed in the cytoplasm of COCs and embryos,which was weaker in the nucleus.Analyzing by immunofluorescence IOD(integrated optical density) showed that the IOD of 9-16-cells embryos was highest, followed by blastocyst, 2-4-cells embryos, which was lowest in 5-8-cells embryos. The results showed that Oct 4 could express in various periods of yak oocytes and embryo development, while the expression levels of Oct 4 were different, which indicated that Oct 4 might participate in the embryonic genetic transcription activation at stages of 9-16-cells, which might take part in maintaining tipotency of inner cell mass in blastocyst.

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